The issue has been in the spotlight recently as Russia prepares to host the 2014 Winter Olympics in Sochi. The International Olympic Committee has said the law won't apply to athletes or spectators at the games.
President Barack Obama met with Russian gay rights activists earlier this month during his trip to the country for the G-20 summit. During the hourlong meeting, Obama said their work was "critically important" to an open society and that he was "very proud" of their efforts.
Asked for an overall response to Putin's op-ed, and whether she thought it was a shot at Obama's handling of the situation in Syria, Pelosi shrugged.
"It is what it is," she said. "Vladimir Putin is not in a strong constitutional democracy where people have their say, so he comes here and has his say."
She added, "But it has to have some fidelity to fact."